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Six simple sequence repeats (SSR) markers were developed from expressed sequence tags (ESTs) in the genus  Larix . Based on evaluation with 49  L. kaempferi  genotypes, the number of alleles per locus ranged from two to four, and the expected ( He ) and observed ( Ho ) heterozygosity values were 0.225−0.694 and 0.201−0.656, respectively. The inbreeding coffcient ( F IS ) for all loci were less than zero except that LAReSSR85 was 0.4383. All the six EST-SSR markers were transferable to  L. gmelini ,  L. olgensis  var  Koreana ,  L. principisrupprechtii  and  L. olgensis . BlastX analysis showed that five of the EST-SSRs were homologous to known genes. The six EST-SSR markers developed here can be valuable for biological applications in  Larix .

Short note: Development of Six EST-SSR Markers in Larch