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In the present work, [ 99m Tc]N-histamine has been labeled using [ 99m Tc≡N] 2+  core. Factors such as, amount of substrate, pH, reaction temperature,  in vitro  stability and reaction time, have been systematically studied to optimize high radiochemical purity (&gt;99±0.3). Biodistribution studies indicate the suitability of [ 99m Tc]N-histamine as a novel tracer to image brain. The complex of [ 99m Tc]N-histamine may be considered a highly selective radiotracer for brain imaging.

Preparation and biological evaluation of 99m Tc N-histamine as a model for brain imaging: in silico study and preclinical evaluation