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The synthesis of 6-azidomethyl-5,6,7,8-tetrahydropterin (6-AzmH 4 Ptr) (9) starting from 2,4-diamino- 6-hydroxymethylpteridine (1) is described. A special protecting group strategy had to be applied to perform the structural modifications. The introduction of the azido function was achieved by a Mitsunobu reaction converting the N 2  - N ,N -dimethylaminomethylen-6 -hydroxymethyl-5-pivaloyl-5,6,7 ,8-tetrahydropterin (7) with diphenylphosphoryl azide into the corresponding 6-azidomethyl derivative (8). The newly synthesized compounds have been characterized by elemental analyses, UV, IR and  1 H-NMR spectra. The photolysis of 6-AzmH 4 Ptr (9) and its inactivation effect upon aromatic amino acid mono oxygenases has been studied

pteridines

Pteridines CVII* Synthesis of 6-Azidomethyl-5,6,7,8-tetrahydropterin. Photo degradation Products and Cofactor Properties on Aromatic Amino Acid Monooxygenases