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The avian eggshell membranes’ composition depicts close resemblance with the extracellular matrix of the cells, and therefore being widely employed as potential biomaterials for tissue engineering applications. However, the optimization of process conditions for collagen extraction, the main constituent of eggshell membranes is still challenging. In the present study, extraction of collagen was performed by an enzymatic method optimized through the one-factor-at-a-time (OFAT) technique for three parameters viz. pepsin concentration, treatment time and pH. The process optimization resulted in the maximum yield of 56% collagen with 350 U/mg pepsin concentration at pH 3 treated for 9 days, not reported yet. The collagen extraction was confirmed by OD at 232 nm; and its viscoelasticity behaviour at pH 5. The physico–chemical characterization of extracted collagen with FESEM, ATR-FTIR, surface roughness analysis and contact angle measurement revealed the morphological and topological alteration during the collagen extraction. The process optimization and characterization of eggshell membrane derived collagen can aid in the significant biomaterials development for tissue regeneration.

Process optimization for extraction of avian eggshell membrane derived collagen for tissue engineering applications