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Novel bio-composite films based on Algerian earth chestnut i.e.  Bunium incrassatum  roots (Talghouda, TG) and cellulose derivatives (ethylcellulose; EC and cellulose acetate; AC) are prepared and tested for methylene blue (MB) adsorption from aqueous solutions. The biomaterial films are elaborated by dissolution solvent evaporation technique and are characterized by infrared spectroscopy, X-ray diffraction, SEM and optical microscopy. The pH pzc  is also determined. For the adsorption tests, design of experiments based on 2 3  factorial design is built and followed. So, the effects of TG:EC:AC ratio, pH and MB initial concentration are discussed on the basis of mathematical modelling using Minitab software. Mathematical relations between equilibrium adsorption percentages and capacities versus selected variables were obtained and illustrated by surface plots. The interactive effects between variables have been also identified. The results showed that the MB adsorption percentage exceeded 83% and is mostly affected by pH value. Nevertheless the adsorption capacity is affected by MB initial concentration.

Design of experiments for the methylene blue adsorption study onto biocomposite material based on Algerian earth chestnut and cellulose derivatives