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Background and objective:  Various optical imaging techniques have shown a great potential for monitoring angiogenesis, development of blood vessels, and even tumor transfer, but they suffer from the limited imaging depth in tissue. Although the mouse ear provides an available window, the residual scattering of ear skin still influences the imaging quality. The proposed tissue optical clearing technique presents a new opportunity to decrease the scattering of skin, and enhance the imaging contrast or imaging depth of optical methods. The purpose of this study is to develop an innovative ear skin optical clearing agent (ESOCA) for improving the transparency of the mouse ear.

Ear skin optical clearing for improving blood flow imaging/Optisches Clearing der Ohrhaut zur verbesserten Bildgebung des Blutflusses