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The purpose of this study was to evaluate the influence of low-level laser therapy (LLLT) on epiphyseal cartilage in the femur and tibia of rabbits. A total of six male New Zealand rabbits were used in this study. The right tibia or femur lesions (control group) served as negative control and the left as experimental (laser) group. Helium-neon (HeNe) laser light with an energy density of 6 J/cm 2  and wavelength of 632.8 nm was applied to the left knees (laser group) over a period of 1, 3 and 5 weeks. All sections were analyzed using hematoxylin and eosin stained sections, and thickness of the resting, proliferative and hypertrophic and degenerative layers were measured. The results showed that the epiphyseal cartilage zones could be easily identified in both groups (control and laser). The measurements of epiphyseal plate showed no statistically significant differences ( p &gt;0.05) between control and experimental groups. Nevertheless, when each zone of the femur and tibia was analyzed, resting and proliferative layers decreased in both bones with laser application, showing statistically significant differences ( p &lt;0.05) for all weeks analyzed at this setting. Taken together, these results suggest that HeNe laser stimulates the cartilage ossification to bone, causing some delay in growth and development of the epiphyseal plate.

photonics and lasers in medicine

Helium-neon laser therapy interferes with epiphyseal plate growth in the femur and tibia of rabbits: Helium-Neon-Lasertherapie interferiert mit dem Epiphysenplattenwachstum im Oberschenkelknochen und Schienbein von Kaninchen