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A label-free angiography photoacoustic microscopy system is proposed which was produced by the integration of a two-dimensional scanning galvanometer, an objective lens, a focused ultrasound transducer, a water container and a sample supporter. Using this system,  in vivo  blood vessels and capillaries in the back of a mouse and in the mouse ear were clearly imaged. Moreover it was possible to reconstruct a three-dimensional image of the mouse back vascular network.  Ex vivo  erythrocytes were used to prove the imaging capability of the system, and a single erythrocyte was mapped with high contrast. The experimental results demonstrate that the galvanometer-based photoacoustic microscopy system can accomplish label-free photoacoustic imaging and that it has clinical potential for use in the detection of erythrocytes and blood vessels.

photonics and lasers in medicine

Photoacoustic microscopy imaging – From a single erythrocyte to a vascular network/Bildgebung mittels photoakustischer Mikroskopie – Vom einzelnen Erythrozyt zum Gefäßnetz