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Bats play an important role by providing ecosystem services including pollination, seed dispersal, forest regeneration and insect pest control and also serve as bio-indicators. In the present study, we present an acoustic guide to the calls of nine species of bats from Gujarat belonging to families Rhinopomatidae ( Rhinopoma hardwickii ,  Rhinopoma microphyllum ), Emballonuridae ( Taphozous melanopogon ,  Taphozous longimanus  and  Taphozous nudiventris ), Rhinolophidaea ( Rhinolophus lepidus ), Hipposideridae ( Hipposideros galeritus ) and Vespertilionidae ( Scotophilus heathii ,  Pipistrellus ceylonicus ). Discriminant function analysis was used to classify the bat calls to the species level using leave-one-out cross validation. Analysis was carried out separately for constant frequency (CF) calls and frequency-modulated (FM) calls. Bats echolocating with CF calls were classified with 100% success, while in the case of FM calls, the calls were classified with 66.7% accuracy. In species-rich communities, care should be taken while using echolocation calls to identify bats producing FM calls. More such call libraries of bats from other parts of India are needed for non-invasive documentation of chiropteran fauna in different biogeographic zones.

Echolocation calls of some bats of Gujarat, India