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Monitor ing of heart-rate variability (HRV) has been clinically used in cardiotochography and in neonatal intensive care. For clinical purposes visual analysis of the instantaneous heart-rate (IHR) curve is often sufficient. However, for quantitative investigation of cardiac control mechanisms more advanced techniques are necessary. For experimental studies in lambs and clinical neonatal monitoring we have developed the following methods: A bipolar electrocardiogram lead was recorded under visual control using a neonatal monitor (Corometrics 512) on analogue magnetic tape by FM tape recorder (Racal 4 ds) . The record was played back at 8 times real-time speed. The R-R intervals were digitized by an interval counter with an accuracy of 1 msec. The signal analysis was performed by digital computer (Nova 3 and Eclipse C/150, Data General Corporation, Southboro, Mass., U S A ) . The R-R interval signal was equispaced by using sin (x)/x digital lowpass f i l ter ing. The cut-off frequency (Fc) was determined on the basis of the longest R-R interval in the original recording. The sampling f requency equaled 2.2 * Fn. LOW-PASS FILTERED SIGNAL AND ITS

ADVANCED QUANTIFICATION OF THE NEONATAL HEART-RATE VARIABILITY BY SPECTRAL ANALYSIS