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The measurement of sample integrity on a clinical laboratory system may help to avoid misleading results. The VITROS ®  5,1 FS Chemistry System is a new analyzer which offers this feature. Approximately 50 diagnostic specimens were evaluated for the presence of hemolysis, icterus, and turbidity using MicroSensor™ technology onboard the VITROS ®  5,1 FS. The samples were also assessed for hemoglobin concentration on a Lange photometer using the Harboe method and values were compared with the MicroSensor™ technology hemolysis index (H-index). For the icterus index (I-index), comparison was based on total bilirubin measured on the VITROS ®  system. To correlate the turbidity index (T-index), a comparison with triglycerides measured on the VITROS ®  5,1 FS was undertaken. In addition, samples run on the VITROS ®  5,1 FS were measured on a manual photometer at 700 nm. Good correlation was established between the manual Harboe method for measurement of hemoglobin and the VITROS ®  5,1 FS H-index. For the I-index, a good correlation was observed compared to the quantitative bilirubin (TBIL slide) measurement. A poor correlation was expected for the comparison of the T-index with the triglycerides as measured on the VITROS ®  system. An additional study comparing the VITROS ®  MicroSensor T-index vs. manual photometer measurement at 700 nm yielded a very good correlation. The VITROS ®  5,1 FS chemistry system MicroSensor™ technology measures the index for hemolyzed, icteric, and turbid samples. The indices correlate well compared to other methods for measuring these indices. Therefore, the use of MicroSensor™ technology offers a useful feature to identify samples containing optical interferents during routine work and to avoid misleading results by providing flags.

Evaluation of sample integrity measurement with VITROS ® 5,1 FS chemistry system / Evaluation der Messung der Probenintegrität am VITROS ® 5,1 FS