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Background: Allo- and autoantibodies against membrane structures of human platelets cause different types of immune thrombocytopenic disorders and require a sensitive and specific assay. The well-established monoclonal antibody immobilization of platelet antigens (MAIPA) test is able to distinguish between specifically bound immunoglobulins and coating of membrane-associated immune complexes; but MAIPA is rather time consuming and requires 2×10 7  platelets or more. For patients with very low platelet count and for little children with small blood volume, this may present a serious limitation.

laboratoriumsmedizin

Differentiation of anti-platelet antibodies using microspheres and flow cytometry Einsatz von Mikrosphären und Durchflusszytometrie für die Differenzierung von Antithrombozyten-Antikörpern