English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Open

Presents the pdf analysis as premium feature

PDF Analysis

Insights

Presents the summarisation as premium feature

Summarisation

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Intracellular recordings were made from neurons of the mouse dorsal cochlear nucleus (DCN) in vitro using current clamp techniques in the presence or absence of different ion channel blocking drugs. Four electrophysiologically distinct cell groups were identified in the DCN. The groups were characterized on the basis of their spontaneous firing properties, the shape of the action potential (AP) and the pattern of firing, the shape of the current-voltage (I/V) relationship and the effects of channel blocking agents. By comparison with known histology, three of the four DCN groups were postulated to be cartwheel-like, fusiform-like, or tuberculoventral-like cells. The fourth group was postulated to be a stellate-like as it had similar properties to the spike train (stellate) cell of the AVCN. DCN stellate-like cells were spontaneously active, the action potentials (APs) were always followed by a large, brief hyperpolarization and the cells had linear current voltage relationships. The fusiform-like cells were spontaneously active and spontaneous IPSPs were also observed. The I/V relationship was linear for these cells. Tuberculoventral-like cells were not spontaneously active, but APs could be elicited by inward current injection. The I/V relationships for tuberculoventral-like cells were linear. Cartwheel-like cells were spontaneously active. These cells were characterized by the distinctive shape of their APs which were single, large amplitude, short duration APs sometimes followed by a series of complexes consisting of small, long duration APs. Cartwheel-like cells were the only cell type in the DCN which had non-linear I/V relationships. All cells in the DCN had APs which were abolished by tetrodotoxin. Different calcium dependent channels play a role in the formation of both the fast single AP and the slow complex AP in the cartwheel-like cells since all APs were abolished by the use of high concentrations of verapamil. Verapamil dramatically increased the duration of APs in fusiform-like cells and had no effect on tuberculoventral-like cells. In both tuberculoventral-like cells and cartwheel-like cells, 4-aminopyridine (4AP) depolarized the cells and all APs were abolished. Tetraethylammonium chloride (TEA) had a similar effect in cartwheel-like cells. In stellate-like, tuberculoventral-like and fusiform-like cells, the hyperpolarization which followed the AP was abolished by TEA. The AP duration in these cells was also increased by TEA. 4AP had a similar effect in stellate-like and fusiform-like cells. The data for DCN suggest that electrophysiological properties can be used to distinguish and identify neurons.

Membrane Properties of Mouse Dorsal Cochlear Nucleus Neurons in Vitro