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Surface mechanical nano-alloying treatment (SMNAT) was employed to fabricate a nanostructured Ti coating on LZ91 Mg–Li alloy. Microstructure, surface hardness and in-vitro biocompatibility of the Ti-coated sample were investigated in comparison with those of an untreated sample. Experimental results showed that a nanostructured Ti coating with a thickness of 35 to 60 μm was formed after SMNAT for 2 h. The average grain size in the top surface of the Ti coating was about 30 nm. The surface of the Ti coating is rougher than that of the untreated LZ91 sample, in which the values of  Ra ,  Rq  and  Rz  were 7.83, 9.57 and 14.85 μm, respectively. The hardness of the Ti coating top surface was about 483 HV. Cell proliferation and differentiation on Ti coated samples were enhanced relative to those on the untreated samples.

Improved in-vitro biocompatibility of LZ91 Mg–Li alloy by formation of nanostructured Ti coating through surface mechanical nano-alloying treatment