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In this study  Ochrobactrum intermedium  (Alhpa-22) was isolated from petroleum refinery sludge and characterized by using 16rRNA. In experimentation, for decolrization of methyl orange dye an indigenous noble bacterial  ochrobactrum intermedium  (Alhpa-22) at 35 °C has been used in a bioreactor. The  ochrobactrum  bacterial decolorized methyl orange dye having the concentration of 50 mgL −1  by 87 % within 9 days, which is a very encouraging result. decolorization of methyl orange dye by the isolated bacteria was elucidated using a UV–Vis spectrophotometer. The experimental data were fitted first and zero order kinetics models which show degradation of dye follows first order kinetics in presence of  Lysinibacillus fusiformis  KLm1, bacterial  ochrobactrum intermedium  (Alhpa-22) and mix consortia of  Lysinibacillus fusiformis  KLm1 and  ochrobactrum intermedium  (Alhpa-22). The rate constant were estimated to be 0.017 h −1 , 0.020 h −1  and 0.014 h −1  respectively. Due to its higher decolorizing capability these may be used for the bioremediation of methyl orange dye (azo dye).

Effect of noble bacteria Ochrobactrum intermedium (Alhpa-22) on decolorization of methyl orange dye in a bioreactor