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Attempts have been made to propose a modified design of inverse fluidized bed bioreactor (IFBBR) that utilizes immobilized enzyme nanosilica particles. The substrate solution is mixed uniformly with nanosilica particles, and the resultant slurry flows down the reactor keeping particles in suspension. The performance characteristics of the bioreactor of proposed design have been analysed both mathematically and experimentally. In the mathematical analysis, dispersed flow of suspension down the reactor tubes has been assumed. The mathematical results (from the developed software package) agree closely with the experimental results, the maximum deviation being 9.5%. The bioreactor provides high fractional conversion at high substrate flow rates, but demands low reactor volume. In addition, the operating cost of the reactor is substantially low. In reactors of this kind, resistance to substrate transfer into the support particles (nanoparticles) is negligible, and the global rate is essentially equal to the intrinsic rate (as the computed value of effectiveness factor is equal to unity for different types of kinetic equations and within wide range of substrate concentration). This must be attributed to the nanosize and consequent large specific surface of support particles. The present design of IFBBR is thus anticipated to be quite attractive for successful industrial adaptation.

Studies on Performance Analysis and Computer Aided Design of Inverse Fluidized Bed Bioreactors with Nanosupport Particles