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A novel method for labelling of anionic groups on wood surfaces was developed and applied to spruce and aspen wood sections in the native state, and after acid washing and alkaline treatment. The metal ions Mg 2+ , Cu 2+ , Sr 2+ , and Zn 2+  were used as markers and their patterns of attachment to anionic groups were assessed by time-of-flight secondary ion mass spectrometry (ToF-SIMS). Sr 2+  was found to be a very suitable marker for labelling of anionic groups. In addition to ToF-SIMS, laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was used for semi-quantitative analysis of labelled anionic groups in wood samples. ToF-SIMS imaging of Sr 2+ -labelled anionic groups revealed abundant anionic groups in bordered pit tori and ray parenchyma cell walls in spruce wood. LA-ICP-MS revealed that the relative concentration of Sr 2+  in tori of bordered pits was approximately eight-fold higher than in fibre cell walls. After alkaline treatment the concentration of anionic groups in the fibre cell wall was approximately three-fold higher than in the original acid-washed spruce. In aspen wood, anionic groups were located mainly in pits between vessels and contact ray parenchyma cells, in ray cell walls and in certain fibre wall layers.

holzforschung

Determination of anionic groups in wood by time-of-flight secondary ion mass spectrometry and laser ablation-inductively coupled plasma-mass spectrometry