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In situ analysis of lignin by 2D NMR of whole plant material was carried out by swelling finely ball-milled samples in deuterated dimethylsulfoxide (DMSO- d 6  ) and sonicated so that a gel was formed in the NMR analysis tube. Solution HSQC NMR spectra of different plant materials representative for hardwood ( Eucalyptus globulus ), softwood ( Picea abies ), and non-woody plants ( Agave sisalana ) are presented here. The spectra show signals corresponding to those of the main plant constituents, such as lignin and polysaccharides. The lignin signals were assigned by comparing the HSQC spectra of the whole plant materials with the HSQC spectra of their respective milled-wood lignins (MWLs). In general terms, the major lignin structural features, such as the relative abundances of the main lignin substructures, the syringyl/guaiacyl ratios and the extent of γ-acetylation of the lignin side-chain observed in the HSQC spectra of the whole plant materials, matched those obtained from the HSQC spectra of the isolated MWLs. Therefore, this technique, which needs only minor amounts of lignocellulosic material and minimal sample preparation, can be useful for the rapid screening of plant lignins without the need for tedious and time-consuming lignin isolation procedures.

holzforschung

HSQC-NMR analysis of lignin in woody ( Eucalyptus globulus  and  Picea abies ) and non-woody ( Agave sisalana ) ball-milled plant materials at the gel state 10 th  EWLP, Stockholm, Sweden, August 25–28, 2008