English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Analyzing the gene sequences of terpene synthase (TPS) may contribute to a better understanding of terpenes biosynthesis and evolution of phylogenetic taxonomy.  Chamaecyparis formosensis  is an endemic and precious conifer of Taiwan. To understand the biosynthesis mechanism of terpenes in this tree, a full length of putative mono-TPS, named as  Cf-Pin  (GeneBank accession no. EU099434), was obtained by PCR method and RACE extension. The  Cf-Pin  has an 1887-bp open reading frame and encodes 628 amino acids. To identify the function of  Cf-Pin,  the recombinant protein from  Escherichia coli  was incubated with geranyl diphosphate, produced one major product, the structure of which was elucidated. GC/MS analysis and matching of retention time and mass spectrum with authentic standards revealed that this product is  α -pinene. This is the first report of cloning of a mono-TPS and functionally expressed in  E. coli  and which could be identified as  α -pinene synthase from a Cupressaceae conifer.

Cloning and characterization of α -pinene synthase from Chamaecyparis formosensis Matsum