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A new method for the isolation of lignin in high yield from wood and pretreated wood is presented, avoiding the liquid-solid extraction step of the classical milled wood lignin (MWL) isolation. Dissolved wood lignin (DWL) was obtained by total dissolution of ball milled wood in dimethylsulfoxide and  N -methylimidazole (DMSO/NMI) followed by precipitation in dioxane/water in the course of which lignin and carbohydrate fractions were separated. The lignin fraction was purified. High lignin yields and the low number of separation steps belong to the advantages of the described method. DWL lignin was isolated from beech wood ( Fagus sylvatica  L.) and the sample was compared to MWL obtained by the classical Björkman method. Elemental analyses, methoxyl group content, potassium permanganate oxidation, infrared spectroscopy, and 1D and 2D nuclear magnetic resonance spectroscopy indicated that both lignin preparations are quite similar. However, MWL contained significantly more phenolic hydroxyl groups than DWL. The results indicated that MWL contains higher amounts of smaller fractions with broken β-O-4 linkages than DWL.

A new and facile method for isolation of lignin from wood based on complete wood dissolution