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In order to establish integrated control strategies of wood degradation, a systematic survey of basidiomycete decay fungi colonizing various wood products in service is a prerequisite to increasing the service life of wood products. As a first step, we initiated a thorough survey of basidiomycete decay fungi colonizing playground wood products. For accurate fungal identification, traditional methods were complemented with molecular methods, including a BLAST search for large-subunit 28S rDNA sequences in Genbank and phylogenetic analysis. A total of 132 basidiomycete fungi, including 30 different fungal taxa, were isolated from 35 playgrounds in 15 different areas. Eight species,  Bjerkandera adusta, Ceriporia lacerata, Gloeophyllum trabeum ,  Peniophora  sp.,  Phanerochaete sordida, Schizophyllum commune, Sisto-trema brinkmannii , and  Trametes versicolor , were predominantly isolated. A combination of traditional and molecular tools allowed a more detailed identification and diversity.

Decay fungi from playground wood products in service using 28S rDNA sequence analysis