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The tolerance of various fungi against copper was examined. For this purpose, we impregnated Norway spruce ( Picea abies ) specimens with two different aqueous solutions: copper(II) octanoate with ethanolamine or copper(II) sulfate (c Cu  = 1.0 × 10 −2  mol/l). Impregnated and unimpregnated test specimens were then exposed to brown rot fungi  Antrodia vaillantii  and  Gloeophyllum trabeum  or to white-rot fungi  Schizophyllum commune  and  Trametes versicolor . After 2, 4, 6 and 12 weeks of exposure Electron Paramagnetic Resonance, Atomic Absorption Spectroscopy and mass loss measurements were performed. The results indicate that  A. vaillantii, G. trabeum  and  T. versicolor  transform copper(II) sulfate in wood into non-soluble, and therefore non-toxic, copper oxalate. The intensity of this reaction depends on the amount of excreted oxalic acid and was the highest for  A. vaillantii  and the lowest for  T. versicolor . In the presence of ethanolamine, formation of insoluble copper oxalate was not possible and therefore, decay could not proceed. The major portion of copper remained in the wood and only minor amounts were in some cases translocated into nutrient media.

Changes of EPR Spectra of Wood Impregnated with Copper-Based Preservatives during Exposure to Several Wood-Rotting Fungi