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Ginkgo biloba  L. saplings were cultivated in an airtight growth chamber with  13 CO 2  for two months. The  13 C ratio of the newly developed xylem region was ca. 85%, evaluated by high lateral resolution secondary ion mass spectrometry and thioacidolysis/GC-MS. Quantitative solid-state  13 C direct polarisation/magic angle spinning (DP/MAS) NMR measurements with high-speed MAS of 70.0 kHz were conducted for cutting-milled wood (CMW), ball-milled wood (BMW), and enzymatically saccharified lignin (EL) samples. In addition, quantitative liquid-state  13 C NMR measurements were carried out for EL in DMSO- d   6 . Major lignin aromatic signals were classified into three groups of aromatic carbons of C–H, C–C, and C–O, and their area ratio was compared within these measurements. EL samples in solid- and liquid-state showed nearly the same results. However, the results for CMW and BMW in solid-state NMR suggest the structural alteration of lignin within the sample preparation procedure, including ball milling.

holzforschung

Evaluation of guaiacyl lignin aromatic structures using  13 CO 2  administered  Ginkgo biloba  L. xylem by quantitative solid- and liquid-state  13 C NMR