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The molar mass (MM) and polydispersity indices (PDI) of acetylated hardwood kraft lignin (Ac-HWKL) and Ac-HWKL fractions were measured by size-exclusion chromatography with multi-angle laser light scattering (SEC-MALLS). The detectors worked at LL 658 nm  and LL 785 nm . The MM of Ac-HWKL measured at 785 nm was much smaller than that measured at 658 nm. The number-average (Mn) and weight-average (Mw) molar masses of Ac-HWKL determined at 785 nm were approximately 6.2 and 6.5 times larger, respectively, than the values estimated using a conventional calibration curve created with authentic polystyrene standards in tetrahydrofuran (THF). Based on the Mw of Ac-HWKL fractions, the Mark-Houwink-Sakurada equation for Ac-HWKL was established to be [ η ]/ml g −1 =0.320 M 0.24  in THF and [ η ]/ml g −1 =0.142 M 0.26  in dimethyl sulfoxide (DMSO). These results demonstrate that Ac-HWKL has a more compact structure in an organic solvent than polystyrene.

holzforschung

Determination of the absolute molar mass of acetylated eucalyptus kraft lignin by two types of size-exclusion chromatography combined with multi-angle laser light-scattering detectors