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Enzymatic grafting of kraft lignin (KL) on wood surfaces is presented as a non-leachable wood protection treatment. Scots pine and European beech mini-blocks were treated with KL solution in the presence of laccase isolated from  Myceliophthora thermophila  and the formation of a stable interaction between wood and lignin was observed. Furthermore, the same strategy was employed to graft KL with the simultaneous entrapment of copper in the polymeric net formed. Enzymatic treatment diminished the leachability of the compounds in accordance with the EN 84 standard. The durability of the leached wood blocks was evaluated by accelerated decay tests. Samples with grafted lignin on the surface lost their antifungal activity in long-term exposure at the concentration tested. This observation is in contradiction to the preliminary tests, where KL seemed to have some biocidal activity. On the other hand, KL grafting in combination with copper entrapment improved the decay resistance and the copper leaching was reduced.

holzforschung

Enzymatic grafting of kraft lignin as a wood bio-protection strategy. Part 2: effectiveness against wood destroying basidiomycetes. Effect of copper entrapment