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A bacterial mixture of  Pseudomonas putida  and  Pseudomonas fluorescens  has recently shown the potential to completely reduce monoterpenes within pine wood particles on a laboratory scale. This bacterial combination was then applied onto pine wood strands to obtain emission-reduced oriented strand boards (OSB) produced on a technical scale. Laboratory tests with bacterial inoculated strands were carried out to optimise parameters such as aeration and incubation time. Residual terpene emissions were measured by solid-phase microextraction/ gas chromatography-mass spectrometry (SPME/GC-MS) analysis. Daily aeration, specific pre-cultivation, and increased inoculum size eventually resulted in a reduction of the major softwood terpenes α-pinene, β-pinene, and Δ3-carene by 60, 70, and 40%, respectively, after only 2 days of incubation. Based on these results, OSB were manufactured from strands after bacterial pre-treatment for 2 or 4 days. As expected, terpene emissions from OSB decreased with increasing incubation time. However, even after only 2 days of incubation, α-pinene and β-pinene emissions were appreciably reduced by 40 and 70%, respectively. The method developed here thus appears to be feasible for industrial application although a further reduction of pre-treatment time would be advantageous. The inoculation step will also have to be adapted for technical implementation into the OSB production process.

Biodegradation of terpenes for emission-reduced oriented strand boards (OSB)