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Dalbergia odorifera  T. Chen is a first-grade state protected plant in China. However, it is difficult to distinguish it from the closely related species  Dalbergia tonkinensis  Prain, which is less important in economic value, by wood anatomical features. In this study, three potential DNA barcode sequences, namely  rpoC 1,  trnH - psbA  and internal transcribed spacer (ITS), were used to differentiate wood of  D. odorifera  from  D .  tonkinensis . The average quantities of DNA extracts from twigs, sapwood and heartwood were 16.3, 11.5 and 6.0 ng mg -1 , respectively. The success rates for polymerase chain reaction (PCR) amplification for three loci, namely ITS,  trnH - psbA  and  rpoC 1, were 62.5, 100 and 81.25%, respectively. The success rate for bidirectional sequencing of amplified products was 100% for all the three loci. The identification power of the three proposed DNA barcodes has been calculated by the BLAST, tree-based method and the TAXONDNA method. The interspecific differences of the  trnH - psbA  region were greater than intraspecific variations. Moreover, the identification power of  trnH - psbA  was higher than that of ITS and  rpoC 1 regions at the species level. Finally, the  trnH - psbA  region is proposed as a DNA barcode for wood identification between  D .  odorifera  and  D .  tonkinensis .

Testing three proposed DNA barcodes for the wood identification of Dalbergia odorifera T. Chen and Dalbergia tonkinensis Prain