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Silver fir and Norway spruce wood have been thermally modified (TMW) for 3–4 h at 160°C, 180°C, 200°C, and 220°C by means of the thermovacuum process (Termovuoto), and the ultrastructural and chemical changes in the compound middle lamella (CML), including the middle lamella cell corner (ML cc ) regions (CML cc ), were investigated. Severe anatomical and histochemical changes were prominent above treatment temperatures of 200°C; thus, woods treated at 220°C for 4 h were in focus. Immunocytochemical studies showed that noncellulosic polysaccharides, such as pectin, xyloglucan, xylan, and mannan, were significantly degraded in CML cc  regions of TMWs. After treatment, the CML cc  regions were composed almost entirely of modified lignin with increased amounts of acidic groups. With cytochemical staining for lignin, many electron dense particulates were detected in the CML cc  regions of TMWs, indicating early degradation/alteration by the Termovuoto treatment.

Chemical and ultrastructural changes in compound middle lamella (CML) regions of softwoods thermally modified by the Termovuoto process