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A sugar solution containing 31 g l -1  xylose was prepared from the culm of  Sasa kurilensis  by hydrolysis with 2% sulfuric acid with a liquor-to-solid ratio of 6 (g g -1 ) at 121°C for 1 h. During acid hydrolysis, also some byproducts were generated, such as acetic acid, furfural, 5-hydroxymethylfurfral, and low molecular weight phenolics, which inhibit bioconversion of xylose to xylitol. Except for acetic acid, these inhibitors were successfully removed from the hydrolysate by contacting with a steam-activated charcoal (15 g l -1  dose) for 24 h. Bioconversion of the detoxified hydrolysate to xylitol by the yeast,  Candida magnoliae , was investigated under various microaerobic conditions. The oxygen transfer rate (OTR) varied from 8.4 to 27.6 mmol-O 2  l -1  h -1 . The maximum xylitol yield (0.62 g-xylitol g-xylose -1 ) was attained at the OTR of 1.2 mmol-O 2  l -1  h -1 . An additional increase in the OTR brought about cell growth, which consumed xylose. A proper control of the oxygen supply is necessary to produce efficiently xylitol from the culm hydrolysate.

Microbial xylitol production from culm of Sasa kurilensis using the yeast Candida magnoliae