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The compound ZKY-1 with a D-π-A structure was designed and synthesized in four steps. It undergoes complexation with a tetravalent zirconium ion and the complex can be used for the detection of phosphorylated proteins. The detection limit is estimated to be 100 n m  in vitro . The successful detection of phosphorylated proteins in placental tissue samples indicates that this probe holds great potential for clinical diagnosis.

A novel molecular probe for the detection of phosphorylated proteins