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The pH fluorescent probe 7-hydroxy-4-methyl-8-(((2-(1-phenyl-1 H -phenanthro[9,10- d ]imidazol-2-yl)phenyl)imino)methyl)-2 H -chromen-2-one (PICO) contains a donor–π–acceptor (D–π–A) conjugated system. The ‘off−on’ probe PICO has a pKa value of 8.01 and its fluorescence intensity is enhanced with increasing pH.

A fluorescent pH probe for an aqueous solution composed of 7-hydroxycoumarin, Schiff base and phenanthro[9,10- d ]imidazole moieties (PICO)