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Cyanuric chloride was allowed to react with  N,N -diethylaniline to obtain 4-(4,6-dichloro-1,3,5-triazin-2-yl)- N,N -diethylaniline, which was converted into six novel 1,3,5-trisubstituted triazine derivatives on reaction with different amino acids. These compounds had UV absorption in the range 352–379 nm, accompanied by intense single emission in the range 420–497 nm with fairly good quantum yield (0.106–0.383). The new compounds were characterized by FT-IR,  1 H NMR,  13 C NMR, mass spectral, and elemental analyses. These fluorophores were conjugated with protein bovine serum albumin through carbodiimide chemistry between the negatively charged carboxylate groups (-COO-) of the fluorophore and the surface terminated positively charged amino groups (-NH 3  + ) of the protein. The interaction between functionalized amino acids with protein molecules was investigated using fluorescence spectroscopy showing fluorescence enhancement or quenching of the fluorophore after conjugation.

heterocyclic communications

Synthesis of novel fluorescent 1,3,5-trisubstituted triazine derivatives and photophysical property evaluation of fluorophores and their BSA conjugates