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The synthesis of silver nanoparticles using plant-based materials has seen a surge in recent years. This study used the  Syzygium aromaticum  (clove) buds extract as a reducing agent for synthesizing silver nanoparticles (Sa-AgNPs). The presence of Sa-AgNPs (440 nm) was confirmed by UV-Vis spectroscopy. The optimization of nanoparticle production with pH, metal ions, and substrate concentration (clove extract) was studied. The transmission electron microscopy analysis revealed that Sa-AgNPs had a size distribution predominantly below the range of 10–100 nm. The investigation of Sa-AgNPs using EDX revealed the presence of an optical absorption silver peak at 3 keV. The involvement of phenolic chemicals and carboxylic acids in stretching O–H, N–O, and C═O bonds, forming Sa-AgNPs has been identified by Fourier transform infrared spectroscopy.  Klebsiella pneumoniae  and  Trichophyton rubrum  exhibited a higher inhibition zone of 26 ± 0.48 mm and 21 ± 0.48 mm in antibacterial and antifungal activity, respectively. In the 2,2-diphenyl-1-picrylhydrazyl experiment, at a maximum concentration of 500 μg·mL −1 , Sa-AgNPs exhibited a scavenging efficiency of 79.98%. Cytotoxicity was observed in the treated cells due to the presence of biologically synthesized Sa-AgNPs. An IC 50  value of 48 μg·mL −1  was determined by treating L929 human fibroblast cells.

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Antibacterial, antifungal, antioxidant, and cytotoxicity activities of the  aqueous  extract of  Syzygium aromaticum -mediated synthesized novel silver nanoparticles