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Extraction with supercritical carbon dioxide (SC-CO 2 ) which is known as a clean technology was carried out to extract oil from melon ( Cucumis melo ) seeds. SC-CO 2  extraction technique does not contaminate extracts. SC-CO 2  is not a toxic and a flammable solvent. Phytosterols, natural and bioactive compounds, which is known to provide protection against various chronic diseases were examined in the seed oil by using gas chromatography – mass spectrometry (GC-MS). Stigmasterol and β-sitosterol were detected in the melon seed oil. SC-CO 2  extractions were performed in a range of 30-55°C, 150-240 bar, 7-15 g CO 2 /min, 0.4-1.7 mm (mean particle size of the seeds) and 1-4 h. The optimal quantities of extracted oil, β-sitosterol and stigmasterol were 36.8 g/100 g seed, 304 mg/ kg seed and 121 mg/ kg seed, respectively, at 33°C, 200 bar, 11 g CO 2 /min, 0.4 mm and 3 h.

Extraction of phytosterols from melon ( Cucumis melo ) seeds by supercritical CO 2 as a clean technology