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In this study, enantiomerically pure ( S )-1-phenylethanol was produced via asymmetric bioreduction of acetophenone. Ispir bean  (Phaseolus vulgaris ) was used as an alcohol dehydrogenase (ADH) source since whole cells are cheaper than isolated enzymes. Acetone powder methodology was applied for biocatalyst. Glucose was used as a cosubstrate in-order to regenerate cofactor (NADPH). The reactions were carried out in an orbital shaker whose temperature and agitation rate can be controlled. ( S )-1-phenylethanol concentration was analyzed by HPLC using a Chiralcel OB column. Effects of the reaction time, substrate concentration, cosubstrate concentration and biocatalyst concentration on the ( S )-1-phenylethanol production were investigated using Response Surface Methodology (RSM). 36 h bioreduction time, 6 mM acetophenone concentration, 25.15 mM glucose concentration, and 175 mg/mL biocatalyst concentration were determined as optimum values. In these conditions, 2.4 mM ( S )-1-phenylethanol was obtained in phosphate buffer (pH=7.0) at 30°C with &gt;99% enantiomeric excess.

Optimization of the asymmetric synthesis of ( S )-1-phenylethanol using Ispir bean as whole-cell biocatalyst