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Production of biodiesel in water/oil (w/o) microemulsion system through a lipase-catalyzed (lipase from porcine pancreas) methanolysis of soybean oil was investigated. The independent factors were researched and response surface methodology (RSM) based on the Box-Behnken design was used to optimize the significant reaction variables, including  w  0  (defined as the molar ratio of water to surfactant), lipase concentration, reaction time and substrate molar ratio. Results indicated that the optimal conditions for biodiesel preparation were:  w  0  3.6, lipase concentration 4.3% (based on oil weight, g), reaction time 17 h and molar ratio (methanol/oil) 10.5. The actual fatty acid methyl ester yield (96.8%) coincided with the optimum predicted value (97.5%) under the optimal conditions. Fatty acid methyl ester synthesized in microemulsions directly which may provided a potential way to produce biodiesel microemulsion.

Optimized microemulsion production of biodiesel over lipase-catalyzed transesterification of soybean oil by response surface methodology