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The influence of the steric exclusion mechanism on the dependence of the retention on the thickness of the micro-TFFF channel was studied theoretically. A good agreement of theory with experimental data was found. In order to preserve the use of micro-TFFF within an extended range of molar masses and particle sizes, the thickness of the channel cannot be reduced without taking into account a possible deterioration of the separation due to the contribution of steric exclusion to the separation mechanism.

Micro-thermal field-flow fractionation in the characterization of macromolecules and particles: Effect of the steric exclusion mechanism