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In this study, the extraction of Glycyrrhizic acid (GA) from Glycyrrhiza glabra (licorice) root was investigated by Soxhlet extraction and modified supercritical CO 2  with water as co-solvents and 30 min of static extraction time. The high performance liquid chromatography (HPLC) was used to identify and quantitatively determine the amount of extracted GA recovery of supercritical CO 2  extraction of GA. The extraction recovery was modeled by adaptive neuro-fuzzy inference system (ANFIS) and artificial neural network (ANN). Different ANFIS networks (by changing the type of membership functions) were compared with evaluation of networks accuracy in GA recovery prediction and subsequently the suitable network was determined. A three-layer artificial neural network was also developed for modeling of GA extraction from licorice plant root. In this regard, different networks (by changing the number of neurons in the hidden layer and algorithm of network training) were compared with evaluation of networks accuracy in extraction recovery prediction. One-step secant back propagation algorithm with six neurons in hidden layer was found to be the most suitable network and the coefficient of determination (R 2 ) was 98.5 %. Gaussian combination membership function (gauss2mf) using 2 membership function to each input was obtained to be optimum ANFIS architecture with mean square error (MSE) of 0.05,0.17 and 0.07 for training, testing and checking data, respectively.

chemical product and process modeling

Artificial Neural Network and Adaptive Neuro-Fuzzy Interface System Modeling of Supercritical CO 2  Extraction of Glycyrrhizic Acid from  Glycyrrhiza glabra L