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The matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF) and micellar electrokinetic chromatography (MEKC) methods were used to identify and quantify five tannins, (+)-catechin, (–)-epigallocatechin, (–)-epigallocatechin gallate, (–)-epicatechin gallate and (–)-epicatechin, from aqueous, ethanolic and acetonic extracts of  Calendula officinalis, Hypericum, perforatum, Galium verum  and  Origanum vulgare . The MALDI-TOF technique was used for screening tannins monomers and oligomers in plant extracts. The sandwich method and matrix 2,5-dihydroxybenzoic acid with a concentration of 10 mg mL –1  in acetonitrile/ultrapure water/trifluoroacetic acid (20 : 80 : 0.1, vol.) were used. The electrophoretic method developed for the separation and quantification of 5 catechins in 15 min exhibited good efficiency and precision, low limits of detection (0.0032–0.0153 μg mL –1 ) and quantification (0.0096–0.0466 μg mL –1 ). The correlation coefficients ( R  2 ) exceeded 0.9986 and the recovery values ranged between 94.25 % and 102.50 %. The present work provides new information on some of the less studied compounds present in plants frequently used in traditional medicine.

Tannins analysis from different medicinal plants extracts using MALDI-TOF and MEKC