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In order to decrease the difficulty in trace mercury determination, an adsorption method for trace mercury based on  Bacillus subtilis  cells was proposed in this article. The adsorption process was characterized by optical microscopy and SEM. The adsorption mechanism was analyzed by IR. The adsorption performance was studied by measuring the concentration of supernate and calculating the adsorption efficiency. When adsorbing Hg 2+ ,  Bacillus subtilis  cells gathered and their structure turned coarse. The IR results illustrated that functional groups bound with Hg for complexation during adsorption.  Bacillus subtilis  completed adsorption for trace Hg 2+  in 15 min. The adsorption efficiency was maintained above 80% under low Hg 2+  concentrations (&lt;200 µg/L). The proposed study illustrates that  Bacillus subtilis  cells are highly efficient and easily obtained material for the adsorption of trace mercury, which shows potential to be further used in the pretreatment of trace Hg 2+  detection.

Study of an adsorption method for trace mercury based on Bacillus subtilis