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The aim of the study was to identify the  Pm2, Pm3a, Pm4b  and  Pm6  genes and to develop multiplex PCR reaction conditions to reduce time and limit analysis costs. The following molecular markers were used for gene identification:  Xcfd81, Whs350  and  Xgwm205  (for  Pm2 ),  Pm3a  (for  Pm3a ),  STS_241  and  Xgwm382  (for  Pm4b ),  NAU/BCDSTS 135-2  (for  Pm6 ). Plant material consisted of 7 popular European wheat varieties from the wheat collection at the Department of Genetics and Plant Breeding of the Poznań University of Life Sciences. The field experiment was established in 2017 and 2018 on 10 m 2  plots in a randomized complete block design in three replicates in the Dłoń Agricultural Experimental Farm of the Poznań University of Life Sciences (51°41’23.835”N 017°4’1.414”E). The analyses demonstrated that the accumulation of all identified  Pm  genes was found in the Assosan variety. The accumulation of the  Pm2, Pm4b  and  Pm6  genes was found in Atomic, Bussard, Lear, Sparta, Tonacja and Ulka varieties. The work also involved developing multiplex PCR conditions for  Xcfd81  and  STS_241  and  Xcfd81  and  Xgwm382  primer pairs, allowing the simultaneous identification of the  Pm2  and  Pm4b  genes.

Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR