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Background:  α-Thalassemia is one of the most commonly inherited single-gene disorders in southern China. It is important to identify non-deletional α-thalassemia in areas where α-thalassemia is prevalent, since non-deletional HbH disease ( --/α T α  or  --/αα T  ) is caused by the interaction of a non-deletional α-thalassemia with α-thalassemia-1 trait ( --/αα ). In this study, we developed an optimized molecular protocol for screening for α-globin gene mutations and validated the feasibility of using it as a rapid detection method.

Screening for mutations in the α-globin genes leading to abnormal hemoglobin variants with high resolution melting analysis