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Background : Tuberculosis (TB) remains a major health problem affecting millions of people worldwide. One-third of the world's population is infected with  Mycobacterium tuberculosis , the etiologic agent of TB. A simple and rapid method to diagnose TB is urgently needed to be developed. The procedure of systematic evolution of ligands by exponential enrichment (SELEX) is a method in which single-stranded oligonucleotides (called aptamers) are selected from a wide variety of sequences, based on their interaction with a target molecule. Aptamers have been used in numerous investigations as therapeutic or diagnostic tools.

The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosis