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Background : Assay of total serum protein by the biuret method calibrated with albumin standards according to the reference method provides results with a positive bias approximately 3%–5% exceeding the total error of 3.4% allowable for total protein in serum analysis made by analysers using two-part reagents and short-term procedures.

Albumin-based or albumin-linked calibrators cause a positive bias in serum proteins assayed by the biuret method