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Background : We required a new genotyping method for the diagnosis of adult hypolactasia, which would allow the simultaneous genotyping of three known polymorphic loci linked to lactose tolerance ( LCT  –13907C&gt;G,  LCT  –13910C&gt;T and  LCT  –13915T&gt;G) in a single PCR/pyrosequencing test run.

clinical chemistry and laboratory medicine

Simultaneous genotyping of the three lactose tolerance-linked polymorphisms  LCT  –13907C&gt;G,  LCT  –13910C&gt;T and  LCT  –13915T&gt;G with Pyrosequencing ™  technology