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Background : Linezolid is an important therapeutic option for the treatment of infections caused by multiresistant Gram-positive bacteria such as vancomycin-resistant  Enterococci  (VRE) and methicillin-resistant  Staphylococcus aureus  (MRSA). However, the clinical benefit of linezolid is threatened by the emergence of resistant strains of MRSA and VRE reported in North America and Europe. For effective antimicrobial treatment, it is extremely important to have exact knowledge of drug concentrations at the site of action.

A simple isocratic HPLC assay to determine linezolid concentrations in different biomatrices for in vivo and in vitro studies