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Background : Cobalamin (Cbl) deficiency is a common clinical phenomenon, in particular among the elderly and possibly also among infants. Methylmalonic acid (MMA) is the most sensitive and specific marker of intracellular Cbl status, but its application is hindered by limited methods available for accurate and high-throughput MMA determination.

A simple high-throughput method for the determination of plasma methylmalonic acid by liquid chromatography-tandem mass spectrometry