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Background : Apolipoprotein E ( APOE ) and choline acetyltransferase ( ChAT ) have been suggested as candidate genes for determining the risk of late-onset Alzheimer's disease. The aim of this study was to simultaneously detect polymorphisms in codons 112 and 158 of  APOE  and codon 2 of  ChAT  by hybridization probe multiplexing.

clinical chemistry and laboratory medicine

Real-time multiplex PCR assay for genotyping of three apolipoprotein E alleles and two choline acetyltransferase alleles with three hybridization probes