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We evaluated the analytical performances of the new Sebia kit for quantification of hemoglobin fractions (HbA, HbF and HbA 2 ) and structural hemoglobin variants on the Capillarys ®  system. This automated capillary zone electrophoresis method uses an alkaline buffer with silica capillaries and spectrophotometric detection. Specimen stability was evaluated during 1 month. The reproducibility of migration and the imprecision of quantification were also investigated. Comparison with the Beckman P/ACE ®  system was performed on 202 samples. A total of 131 subjects without any hematological abnormality were analyzed to establish the HbA 2  reference ranges based on our local population. Quantification of the Hb fractions and variants exhibited excellent stability for 4weeks of storage at 4°C, with CVs &lt;0.3%. The imprecision of the migration normalized to that of HbA 2  for all hemoglobins tested (fractions and variants) was low, with a CV of &lt;2.5%. At physiological and pathological levels, total imprecision ranged from 1.9% to 4.6% for HbA 2 , from 0.6% to 9.7% for HbF, and from 0.6% to 1% for HbS. Statistical analysis revealed a small proportional negative bias for HbA 2  (−8.6%). Small systematic bias (−0.2%) and proportional bias (−28%) were observed for HbF. No statistically significant difference was found for HbS. The reference range for HbA 2  was 2.1–3.2%. The Capillarys ®  system is a fully automated and accurate system that gives high-resolution performance and displays appropriate characteristics for use as a routine method for the diagnosis of thalassemias and hemoglobinopathies.

Evaluation of a new Sebia kit for analysis of hemoglobin fractions and variants on the Capillarys ® system