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C-Reactive protein has been associated with several complications of pregnancy. The aims of the present study were: (1) to evaluate a turbidimetric immunoassay for the measurement of C-reactive protein; and (2) to investigate the chronological changes of the levels of this protein from preconception throughout normal pregnancy and its relationship with variables associated with preconception and pregnancy outcome. Inter-assay imprecision was &lt;5% for C-reactive protein &gt;1mg/L and 18% at a mean value of 0.33mg/L. The limit of detection was 0.10mg/L. The method was linear between 0.10 and 30mg/L. There were no observed interferences from jaundice, hemolysis, lipemia or paraproteinemia at the levels studied. There was good agreement with the nephelometric method. A total of 39 women were studied at preconception, at 8, 20 and 32weeks of pregnancy, and in labor. Preconception C-reactive protein concentration was 1.17±0.18mg/L and increased (p&lt;0.001) throughout pregnancy up to 5.69±0.82mg/L. Body mass index at preconception and weight gain during pregnancy were the main factors associated with this increase in C-reactive protein.

clinical chemistry and laboratory medicine (cclm)

Evaluation of a high-sensitivity turbidimetric immunoassay for serum C-reactive protein: application to the study of longitudinal changes throughout normal pregnancy